Reagents for sequencing in MiSeq and MiniSeq platforms
Inactive on SAM.gov — responses were due 29 Jul 2026 · 8(a) Sole Source (FAR 19.8) · Department of the Navy, DoD · CALLAO, PE-CAL · NAICS 424210 · Solicitation VEID260013
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SAM.gov marked this notice inactive — responses were due 29 July 2026.
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Description
PERFORMANCE WORK STATEMENT SCOPE The laboratory of the Virology Department of the U.S. Naval Medical Research Unit SOUTH (NAMRU SOUTH) is looking to purchase reagents to prepare libraries using human samples that later will be sequenced in the MiSeq and MiniSeq platforms developed by Illumina, as part of the projects of next-generation sequencing (NGS) that are being conducted at this command. BACKGROUND The kits must be designed for library preparation and sequencing. The library preparation kit should include a variety of steps that include enzymatic fragmentation, size selection, end-repair, a-tail and ligation step in the same reaction. The process should allow including dual index at the 5 and 3 prime ends for later sample identification. Also, the library preparation kit should be designed to construct libraries with a low DNA input (~50ng) with master-mixed reagents ready to use. Similarly, the sequencing kit should allow us to run more than 150 sequencing cycles. Those cycles are needed to run paired-end libraries with a high-quality performance. We expect to have more than 2.0Gb per sequencing run with high Q30 values. Finally, the sequencing kit should include an internal control to validate the sequencing run. REQUIREMENTS Location of work / Place of delivery Shipment to NAMRU SOUTH Callao, Peru Period of performance / Delivery time In accordance with deliverables schedule. Deliverables schedule (if applicable) Materials should be shipped upon award signature. Hazardous material category. None of the items are hazardous materials. List of products FC-110-3001 PhiX control v3 kit MS-102-3003 MiSeq Reagent Kit v3 (600-cycle) FC-131-1096 Nextera XT DNA Sample Preparation Kit FC-131-2001 Nextera XT Index Kit v2 Set A (96 indexes, 384 samples) FC-420-1003 MiniSeq High Output Reagent Kit (300-cycles) FC-131-2002 Nextera XT Index Kit v2 Set B (96 indexes, 384 samples) Applicable Standards These reagents are very time-sensitive and have limited expiration dates, reason for which, vendor should inform final users of expiration dates as soon as they become available and request their approval prior to shipment. Special Requirements All materials require cold chain.
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